Journal: Reproduction & Fertility
Article Title: Artificial intelligence model for the assessment of unstained live sperm morphology
doi: 10.1530/RAF-25-0014
Figure Lengend Snippet: Images captured using a confocal laser scanning microscope (LSM 800). (A) Images captured using a confocal laser scanning microscope (LSM 800) at 40× magnification in the confocal mode (LSM, Z-stack). (Alt-text: series of images captured with a confocal laser scanning microscope at 40× magnification, showing a Z-stack of images in confocal mode. The images provide detailed views of the specimen’s structure). (B) Five zoomed-in frames of sperm with normal morphology. (Alt-text: five close-up images of sperm with normal morphology, displaying their typical structure, including the head, midpiece and tail. The frames emphasise the standard appearance of these sperm features).
Article Snippet: At least 200 sperm were evaluated under 100× magnification using a compound light microscope (ECLIPSE E200; Nikon, Japan).
Techniques: Laser-Scanning Microscopy